The series stars Matthew Morrison, Lea Michele, Cory Monteith, Jane Lynch, Chris Colfer, Amber Riley, Jayma Mays, Naya Rivera, Chord Overstreet, Kevin McHale, Heather Morris, Jenna Ushkowitz, Dianna Agron, Darren Criss, Harry Shum, Jr, Jessalyn Gilsig, Mike O'Malley, and Mark Salling.

Guest stars who have appeared on the show this season inclue Carol Burnett, Britney Spears, Gwyneth Paltrow, Cheyenne Jackson, John Stamos, and Charice. Former guest star Kristin Chenoweth is expected to return.