Glee star Jane Lynch will join the Broadway star in his April performance.
The show will take place tonight at Rockwell Table & Stage.
Tony winner Casey Nicholaw directs the production, with music direction by Rob Berman.
The new musical welcomed Tina Fey and Disney family members including John Lasseter, Bob Iger, Laura Michelle Kelly, and Sierra Boggess.
The musical will also feature Heidi Blickenstaff, Jay Armstrong Johnson, and Jessica Molaskey.
The annual Hollywood event will raise money for AIDS Project Los Angeles.
Little Me with Christian Borle, The Most Happy Fella with Laura Benanti, and John Doyle's Irma La Douce are part of the newest season of the acclaimed concert musical series at New York City Center.
The Broadway heartthrob will make his first-ever appearance at the San Francisco nightclub.
The concert marks Jackson's Birdland debut.