Suzanne Vega, Annie Golden, Chip Zien, and Amy Spanger will also perform at the 54 Below event.
O'Hurley reprises his performance as silver-tongued lawyer Billy Flynn.
Check out photos of the Broadway veterans in performance at swanky nightclub 54 Below.
The musical also welcomed a host of other alums to mark the milestone occasion.
The nightclub has announced special programming for December and January.
Spanger made her Broadway debut in the role in 2002.
The star-studded WTF Late Night Cabaret Series runs through Saturday, January 18.
Broadway's summer home stops in New York for the winter.
The event, which will take place at 54 Below, will also feature Lauren Molina, Todd Almond, and Amy Spanger.